Register now After registration you will be able to apply for this opportunity online.
This opportunity is not published. No applications will be accepted.
Expression analysis of self-incompatibility genes in Lolium perenne L.
The project aims at extending the knowledge of SI in Lolium perenne by quantifying the expression of SI and SC genes in specific tissues.
Sexual reproduction in plants is based on the recognition and compatibility of the pollen (male component) and the stigma (female component). Many angiosperms have developed an incompatibility to self-pollen, preventing selfpollination.
Self-incompatibility (SI) is a genetically mechanism preventing such self-pollination. In grass species, SI is known to be governed by two multi-allelic loci, namely S and Z. Each locus is thought to contain two genes, one expressed in the pollen and the other one in the stigma. However, not much more is known about these genes, their
expression and their interplay in the cascade reaction upon pollen recognition. Moreover, other genes can also prevent self-pollen rejection, leading to self-compatibility (SC). Therefore, we would like to study the expression of
the genes at both S and Z in order to improve our understanding of this SI mechanism, but also at additional loci, known to be involved in (SC).
Sexual reproduction in plants is based on the recognition and compatibility of the pollen (male component) and the stigma (female component). Many angiosperms have developed an incompatibility to self-pollen, preventing selfpollination. Self-incompatibility (SI) is a genetically mechanism preventing such self-pollination. In grass species, SI is known to be governed by two multi-allelic loci, namely S and Z. Each locus is thought to contain two genes, one expressed in the pollen and the other one in the stigma. However, not much more is known about these genes, their expression and their interplay in the cascade reaction upon pollen recognition. Moreover, other genes can also prevent self-pollen rejection, leading to self-compatibility (SC). Therefore, we would like to study the expression of the genes at both S and Z in order to improve our understanding of this SI mechanism, but also at additional loci, known to be involved in (SC).
In this project, we would like to extend our knowledge of SI in Lolium perenne by quantifying the expression of SI and SC genes in specific tissues.
In this project, we would like to extend our knowledge of SI in Lolium perenne by quantifying the expression of SI and SC genes in specific tissues.
This project will study the gene expression of several genes at S and Z, as well as other loci that are known to be involved in SI breakdown. To do so, pollen and stigma samples at different maturity stages will be collected in order to look at the
expression profile of approximately 6-10 genes using qPCR approach.
This project will study the gene expression of several genes at S and Z, as well as other loci that are known to be involved in SI breakdown. To do so, pollen and stigma samples at different maturity stages will be collected in order to look at the expression profile of approximately 6-10 genes using qPCR approach.
You will learn several molecular work technics such as qPCR, RNA extraction, Sanger sequencing and sub-cloning.
You will also learn how to design an experiment to study gene expression, with the statistical analysis of the results.
You will learn several molecular work technics such as qPCR, RNA extraction, Sanger sequencing and sub-cloning. You will also learn how to design an experiment to study gene expression, with the statistical analysis of the results.
A motivated bachelor student with basic skills of molecular work, interested in genetic and gene expression. Being not allergic to pollen is a very important aspect as tissue sampling will be part of the project.
A motivated bachelor student with basic skills of molecular work, interested in genetic and gene expression. Being not allergic to pollen is a very important aspect as tissue sampling will be part of the project.
For any questions or details, please contact Dr. chloe.manzanares@usys.ethz.ch or Prof bruno.studer@usys.ethz.ch
For any questions or details, please contact Dr. chloe.manzanares@usys.ethz.ch or Prof bruno.studer@usys.ethz.ch