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Mapping bone cells in vivo within their 3D environment
Bone remodeling is a dynamic process that requires coordinated interactions between different bone cell types. In order to further our understanding in bone metabolism, these cellular networks and their micro-environments must be investigated locally in a site specific manner.
Keywords: bone remodeling, immunofluorescence, microscopy, gene expression analysis, micro-CT
The skeleton is a metabolically active organ that undergoes continuous remodeling throughout life, a process that is critical to maintain healthy and functional bone. This dynamic process of remodeling consists of coordinated cellular events during which bone resorbing cells (osteoclasts) and bone forming cells (osteoblast lineage cells) interact. Herein, the differentiation of both cell types is controlled in a time and site specific manner. In order to further our understanding of bone metabolism, these cellular networks and their micro-environments must be investigated _in vivo_ at the cellular level.
With the above in mind, the goal of this project is to map the location of specific cell types (osteoblasts, osteoclasts, osteocytes) within mouse bone. Once established, the specific subpopulations of cells from bone can be used to determine expression profile differences as a function of location within bone. Ultimately, this will provide a methodology to investigate how cellular processes are choreographed in space and time in whole organs.
The student will establish and apply histological staining methods to enable the labeling of multiple bone cell types on histological sections from mouse bone. Particularly students with interest and experience in biology and biomedical engineering are encouraged to apply. Please contact the publisher of this document if you would be interested in starting a project in this exciting field of research.
The skeleton is a metabolically active organ that undergoes continuous remodeling throughout life, a process that is critical to maintain healthy and functional bone. This dynamic process of remodeling consists of coordinated cellular events during which bone resorbing cells (osteoclasts) and bone forming cells (osteoblast lineage cells) interact. Herein, the differentiation of both cell types is controlled in a time and site specific manner. In order to further our understanding of bone metabolism, these cellular networks and their micro-environments must be investigated _in vivo_ at the cellular level. With the above in mind, the goal of this project is to map the location of specific cell types (osteoblasts, osteoclasts, osteocytes) within mouse bone. Once established, the specific subpopulations of cells from bone can be used to determine expression profile differences as a function of location within bone. Ultimately, this will provide a methodology to investigate how cellular processes are choreographed in space and time in whole organs. The student will establish and apply histological staining methods to enable the labeling of multiple bone cell types on histological sections from mouse bone. Particularly students with interest and experience in biology and biomedical engineering are encouraged to apply. Please contact the publisher of this document if you would be interested in starting a project in this exciting field of research.
The aim of this project is to establish and apply protocols for multiplexing immunohistochemical and -fluorescence staining of multiple cell types on murine bone tissue sections. Using in-house registration techniques, these histological sections can be registered into the three-dimensional (3D) bone volume obtained by micro-CT. Ultimately, this will allow the mapping of different bone cells within their 3D environment.
The aim of this project is to establish and apply protocols for multiplexing immunohistochemical and -fluorescence staining of multiple cell types on murine bone tissue sections. Using in-house registration techniques, these histological sections can be registered into the three-dimensional (3D) bone volume obtained by micro-CT. Ultimately, this will allow the mapping of different bone cells within their 3D environment.
Ariane Scheuren, arianesc@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller
Ariane Scheuren, arianesc@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller