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3D bioprinting of magnetic nanocomposite cell-laden scaffolds for bone regeneration
In this study, 3D magnetic nanocomposite cell-laden scaffolds will be fabricated by 3D bioprinting in order to study the influence of magnetic nanoparticles (MNPs) on cell viability, cell proliferation and osteogenic differentiation.
Keywords: 3D bioprinting, magnetic nanoparticle, alginate, bone regeneration
The activation of the mechanotransduction signaling pathways in the cells through the application of magnetic forces and MNPs, known as “magnetic actuation”, has been reported to induce stem cell differentiation towards the osteogenic lineage. Studies demonstrated that MNPs exhibited osteoinduction even without external magnetic force. Wu et al. demonstrated that the introduction of magnetic nanoparticles to Ca-P bioceramics could stimulate osteoblast cells adhesion, proliferation, and differentiation in vitro and promote bone formation and growth in vivo. However, MNPs containing biomaterials usually meet inhomogeneous distribution problem, which induce inhomogeneous magnetic effect on cells. Recent advances in 3D bioprinting provide a promising approach to allows precise placement of nanoparticles, cells and hydrogel in a layer-by-layer process. In this project, we will firstly prepare the homogeneous distribution MNPs solution and then add algiante/gelatin powder, according to our previous study, to generate the homogenous MNPs nanocomposite ink. Cells are encapsulated into the ink and 3D magnetic cell-laden scaffolds should be promising fabricated by 3D bioprinting technical.
The activation of the mechanotransduction signaling pathways in the cells through the application of magnetic forces and MNPs, known as “magnetic actuation”, has been reported to induce stem cell differentiation towards the osteogenic lineage. Studies demonstrated that MNPs exhibited osteoinduction even without external magnetic force. Wu et al. demonstrated that the introduction of magnetic nanoparticles to Ca-P bioceramics could stimulate osteoblast cells adhesion, proliferation, and differentiation in vitro and promote bone formation and growth in vivo. However, MNPs containing biomaterials usually meet inhomogeneous distribution problem, which induce inhomogeneous magnetic effect on cells. Recent advances in 3D bioprinting provide a promising approach to allows precise placement of nanoparticles, cells and hydrogel in a layer-by-layer process. In this project, we will firstly prepare the homogeneous distribution MNPs solution and then add algiante/gelatin powder, according to our previous study, to generate the homogenous MNPs nanocomposite ink. Cells are encapsulated into the ink and 3D magnetic cell-laden scaffolds should be promising fabricated by 3D bioprinting technical.
The aim of this study is to fabricate the homogeneous 3D MNPs cell-laden scaffolds.
The aim of this study is to fabricate the homogeneous 3D MNPs cell-laden scaffolds.
Jianhua Zhang, Jianhua.zhang@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller
Jianhua Zhang, Jianhua.zhang@hest.ethz.ch, Institute for Biomechanics, ETH Zürich, Professorship Ralph Müller