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Osteoclastogenesis in 3D bioprinted tissues
3D bioprinting is an emerging technology that open new perspectives for tissue/organ biofabrication but also for biofabrication of relevant in vitro models to study tissue physiology. Printing properties and the physico-chemical properties of the ink must support cell survival and functionality.
Keywords: 3D bioprinting, osteoclasts, cell biology, molecular biology, histology
Bioprinting is an emerging technology that allows 3D control of spatial placement of cells, matrix material and bioactive molecule. Therefore, 3D-bioprinting is an attractive tool towards development of bone tissue fabrication or bone tissue models. The most common technic for bioprinting bone is using extrusion technique. However, the shear stresses produced through the nozzle during during the deposition process might negatively affect the cells, especially for certain type of cells such as primary monocytes. Consequently, bioprinting settings and the ink compositions represent critical factor as they will determine the printability of the construct and thus the 3D-architectural properties of the tissue but as well the cell survival. Furthermore, the ink properties will also dictate the cell fate and in overall the functionality of the tissue. Different hydrogels polymers (such as gelatine, alginate, gelatine/chitosan) have already been investigated to produce engineered bone tissues. In our group we have recently used an alginate/gelatine bioink to print human mesenchymal stem cells and generate bone tissue engineered constructs. Work done has resulted in cell proliferation and ECM formation and mineralization in cell-laden alginate-gelatin bioinks.
Bone is a dynamic tissue that suffers a continuous process of bone formation and bone resorption. This process is directly mediated by hMSC/osteoblast (bone forming cells), osteoclasts (bone resorbing cells). For the establishments of relevant bone tissue organoids there is a need is using multicellular systems.
Bioprinting is an emerging technology that allows 3D control of spatial placement of cells, matrix material and bioactive molecule. Therefore, 3D-bioprinting is an attractive tool towards development of bone tissue fabrication or bone tissue models. The most common technic for bioprinting bone is using extrusion technique. However, the shear stresses produced through the nozzle during during the deposition process might negatively affect the cells, especially for certain type of cells such as primary monocytes. Consequently, bioprinting settings and the ink compositions represent critical factor as they will determine the printability of the construct and thus the 3D-architectural properties of the tissue but as well the cell survival. Furthermore, the ink properties will also dictate the cell fate and in overall the functionality of the tissue. Different hydrogels polymers (such as gelatine, alginate, gelatine/chitosan) have already been investigated to produce engineered bone tissues. In our group we have recently used an alginate/gelatine bioink to print human mesenchymal stem cells and generate bone tissue engineered constructs. Work done has resulted in cell proliferation and ECM formation and mineralization in cell-laden alginate-gelatin bioinks. Bone is a dynamic tissue that suffers a continuous process of bone formation and bone resorption. This process is directly mediated by hMSC/osteoblast (bone forming cells), osteoclasts (bone resorbing cells). For the establishments of relevant bone tissue organoids there is a need is using multicellular systems.
The overall aim of this project is to go one step further and to study the osteoclastogenesis process into a 3D bioprinted osteoclast cells. For this purpose isolation and culture of human monocyte cells from human buffy coats will be performed. Human monocyte cell will be 3D bioprinted and the cell viability rate to the 3D bioprinting process will be evaluated. Evaluation of the ability of human monocytes to differentiate into mature osteoclast cell will be investigated. By immunostaining and real time RT PCR. Task: 70% laboratory work, 20% data analysis, 10% report and presentation.
The project description can be subjected to changes based on the progress stage of our current research and also adapted to the interest and background profile of the student.
The overall aim of this project is to go one step further and to study the osteoclastogenesis process into a 3D bioprinted osteoclast cells. For this purpose isolation and culture of human monocyte cells from human buffy coats will be performed. Human monocyte cell will be 3D bioprinted and the cell viability rate to the 3D bioprinting process will be evaluated. Evaluation of the ability of human monocytes to differentiate into mature osteoclast cell will be investigated. By immunostaining and real time RT PCR. Task: 70% laboratory work, 20% data analysis, 10% report and presentation.
The project description can be subjected to changes based on the progress stage of our current research and also adapted to the interest and background profile of the student.
If you are interested please send an e-mail with a short description of your interests and laboratory experience to: marina.rubert@hest.ethz.ch
Our work on Tissue engineering and Regeneration: http://www.bone.ethz.ch/research/tissue-engineering-and-regenerative-medicine.html
Our lab: http://www.bone.ethz.ch/the-group.html
Professorship: Ralph Müller
If you are interested please send an e-mail with a short description of your interests and laboratory experience to: marina.rubert@hest.ethz.ch
Our work on Tissue engineering and Regeneration: http://www.bone.ethz.ch/research/tissue-engineering-and-regenerative-medicine.html Our lab: http://www.bone.ethz.ch/the-group.html