Functional Immune Repertoire AnalysisOpen OpportunitiesThe immune system plays a crucial role in protecting our body from foreign threats and infections. However, due to ineffective vaccines and pathogens evading the immune system this does not always work which results in many different disease such as chronic inflammation, Cancer, Virus infections. In order to find out why immune cells in our body are not capable of responding efficiently to identifying these threat, we are trying to uncover how each cell behaves when an immune response is triggered. We are doing this by encapsulating cells into individual droplets and observing their behaviour, such as the production of an antibody against a disease. Using this single-cell droplet technology approach, we are measuring in real-time from 10-100,000 cells for multiple of these functions such as Antibodies simultaneously creating large image datasets. In order to improve biology and medicine of the immune systems and the development of better vaccines, we need highly-trained algorithms to better extract relevant conclusions from the big data we generate from highly parallelized, multiplexed single cell experiments. To achieve this, we aim to further improve machine learning and objection detection as well as tracking based algorithms.
- Biomechanical Engineering, Data Structures, Diagnostic Applications, Image Processing, Modeling and Simulation, Neural Networks, Genetic Alogrithms and Fuzzy Logic, Pattern Recognition, Simulation and Modelling, Software Engineering
- Bachelor Thesis, Course Project, ETH Zurich (ETHZ), Internship, Semester Project
| This project uses droplet based microfluidics to encapsulate single-cells for antibody capture, characterization and B cell isolation. The current project involves cloning and expression of novel antigen into an antigen-library for antigen mapping at single-droplet resolution. The project is suited for Master projects and master thesis (>3 months) and involves, cloning, validation, expression analysis as well as single-droplet verification. Finally, antibody secretion experiment are planned to isolate single-antigen binders and B cell sequencing.
Current antigens revolve around the SARS Cov2 Nucleocapsid protein, RBD and envelope proteins to assess antibody cross-reactivity. - Biochemistry and Cell Biology, Biomedical Engineering, Genetic Immunology, Microbiology
- Bachelor Thesis, ETH Zurich (ETHZ), Internship, Semester Project
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